EXPERIMENTAL BIOMEDICAL RESEARCH 2019-07-10T17:42:56+00:00 Prof. Dr. Hayrettin Ozturk Open Journal Systems <p>Experimental Biomedical Research is focused on priority novel research results covering a wide range of experimental and clinical fields in the biomedical sciences</p> Comparison of platelet volume indices in acute coronary syndrome 2019-06-30T11:29:42+00:00 Asli Kurtar Mansiroglu Isa Sincer Yilmaz Gunes <p><strong>Aim: </strong>To compare the parameters showing the platelet volume index in unstable angina pectoris (USAP), non-ST elevation myocardial infarction (non-STEMI) and ST elevation myocardial infarction (STEMI).</p> <p><strong>Methods: </strong>The platelet volume indices of 94 USAP, 161 non-STEMI and 86 STEMI cases with a total of 341 patients (245 men, 96 women) were compared. The patients between March 2015 and October 2018 who admitted to Bolu Abant İzzet Baysal University Hospital with the diagnosis of acute coronary syndrome were compared using platelet distribution width (PDW), PDW to platelet ratio (PPR), mean platelet volume (MPV), MPV to platelet ratio (MPR).</p> <p><strong>Results: </strong>No significant difference was found between the 3 groups in terms of PDW (p = 0.26), PPR (p = 0.87), MPV (p = 0.41) and MPR (p = 0.78) values.</p> <p><strong>Conclusion</strong>: In our study, there was no statistically significant difference between the types of acute coronary syndrome and platelet volume indices.</p> 2019-06-28T20:16:08+00:00 ##submission.copyrightStatement## Effects of magnesium sulphate on liver ischemia/reperfusion injury in a rat model 2019-07-01T08:03:03+00:00 Kerem Akkoca Hamit Yoldas Mustafa Sit Ibrahim Karagoz Isa Yildiz Abdullah Demirhan Murat Bilgi Ozgur Mehmet Yis Aysel Kukner Ayhan Cetinkaya Oguz Catal Bahri Ozer <p><strong>Aim</strong>: To investigate the protective efficacy of magnesium sulphate in a model of rat liver ischemia-reperfusion (I/R) injury.</p> <p><strong>Method: </strong>32 adult female Wistar-Albino rats (250 to 350 g) were used in this experimental study. Rats were divided into 4 groups according to liver ischemia and magnesium sulfate application methods. Group 1 (C); control, group 2 (M); magnesium sulphate, group 3 (I/R); liver I/R, group 4 (I/R+M); I/R + magnesium sulphate treated. The blood samples were centrifuged for the study of aspartate aminotransferase (AST), alanine aminotransferase, prothrombin time (PT), international normalized ratio (INR) troponin I, total antioxidant status (TAS), total oxidant status (TOS) assays. The livers of the animals were removed at the end of the study and samples were taken for histopathological examination.</p> <p><strong>Results: </strong>AST and INR values were significantly decreased in I/R+M group compared to I/R group. There was no significant difference in ALT values of the groups. Although not statistically significant, the TAS values were increased in I/R + M group compared to I/R group rats. In addition, the value of TOS was found to be lower in I/R + M group rats. In the histopathological examination, the mean values of apoptosis and necrosis were lower in the IR+M group compared to the IR group.</p> <p><strong>Conclusion</strong>: The main finding of the present study suggested that magnesium sulphate pretreatment moderately decreased the liver damage through its anti-inflammatory and anti-oxidant effects in a rat model of liver I/R.</p> 2019-06-30T09:06:12+00:00 ##submission.copyrightStatement## A retrospective evaluation of patients with myelodysplastic syndrome 2019-06-30T11:29:43+00:00 Taner Kaya Mehmet Turgut <p><strong>Aim:</strong> To determine our results by examining patient's files who we followed up by myelodysplastic syndrome (MDS) diagnosis between 2005 and 2009 as retrospectively and to compare the accordance of our results with literature.</p> <p><strong>Methods:</strong> We examined 55 patient's files who got MDS diagnosis in 4 year-term. Complete blood count, biochemical analysis, peripheral spread, bone marrow aspiration, bone marrow biopsy examinations, cytogenetic and fluorescence&nbsp;in situ&nbsp;hybridization (FISH) analyses were made for all cases.</p> <p><strong>Results:</strong> Our patient's age average was 69 and when classified according to WHO criterions at the diagnosis time, % 13 (23,6) of cases got diagnosed with RA-RARS, % 29 (52,7) with RCMD-RS, %5 (9,1) with RAEB-1, % 4 (7.3) with RAEB-2, % 3 (5.5) with MDS 5q and % 1 (1.8) with secondary MDS. % 52 of the patients had normal cytogenetic structure. No relation was determined between patients’ diagnosis and international prognostic scoring system (IPSS) scores. During the 4 year-term, our 13 patients died. 6 of these cases died by reason of transforming to acute myeloid leukemia (AML), and 7 patients died because of infection. Mean survival time of cases that died was 6 (1-7) months as of the diagnosis date. Whereas 8 cases were over 70 ages, 5 of our cases were under 70 ages.</p> <p><strong>Conclusion:</strong> MDS is a disease that ranges between anemia and AML and requires cytogenetic trials for diagnosis along bone marrow aspiration and biopsy for the purpose of determining the treatment regimen and prognosis. Determining the IPSS scores of patients by obtained outcomes is required. Risk of transforming to acute leukemia and susceptibility to infection are important in terms of mortality. Overage of patient population restricts the treatment regimens. Age must be an important factor for prognosis and treatment choice.</p> <p>&nbsp;</p> 2019-06-30T10:12:04+00:00 ##submission.copyrightStatement## The role of performing a routine four-quadrant cervical biopsy in patients with negative colposcopic findings in increasing the identification rate of cervical intraepithelial neoplasms 2019-07-10T17:42:56+00:00 Caglar Cetin Sevinc Rabia Serindag Mehmet Ata Topcuoglu Mustafa Ayhan Ekici <p><strong>Aim: </strong>In patients with high-risk human papilloma virus (HPV), there is no consensus on the inclusion of cervical biopsy for diagnostic purposes in cases whereas there is no pathological finding in colposcopy. In this study, we aimed to investigate the effect of simultaneous routine cervical biopsy in patients with normal colposcopic findings on the rate of cervical intraepithelial neoplasia diagnosis.</p> <p><strong>Methods:</strong> This retrospective study included 119 patients with colposcopy indications who had no cervical pathology between January 2015 and March 2017 and the histopathological results were evaluated.</p> <p><strong>Results:</strong> The mean age of the population was 45.75±9.52 years. The histopathological results obtained in our study patients are as follows; 38.7% (n=46) LSIL, 28.7% (n=33) chronic cervicitis, 15, 3 % (n=19) coilositosis, 9.2% (n=11) HSIL, 2.5% (n=5) adenocarcinoma, 1.7% (n=2) carcinoma in situ and 2.1% (n=3) squamous carcinoma. LSIL 33 (27.5%), HSIL and advanced lesion 11 (9.2%) were detected in patients with normal cervical cytology before colposcopy. LSIL 26 (21.7%), HSIL and advanced lesion were found to be 13 (10.8%) in patients with abnormal cervical cytology. There was no significant difference in terms of biopsy pathology results between normal and non-normal cervical cytology results.</p> <p><strong>Conclusions:</strong> In patients with HPV positive and normal colposcopic findings, adding simultaneous routine four-quadrant cervical biopsy to the colposcopy might increase the detection rate of cervical intraepithelial lesions.</p> <p>&nbsp;</p> 2019-06-30T11:10:21+00:00 ##submission.copyrightStatement## Thyroid fine needle aspiration reporting rates and outcomes before and after Bethesda implementation: A single-center experience over 8 years 2019-06-30T11:29:44+00:00 Ismail Alper Tarim Bekir Kuru Kagan Karabulut Gokhan Selcuk Ozbalci Murat Derebey Cafer Polat Aysegul Atmaca Kenan Erzurumlu Bulent Koca <p><strong>Aimː</strong> To evaluate data from our hospital system before and after the implementation of the Bethesda System for Reporting Thyroid Cytology (TBSRTC) and comparison of our data with the previously published studies.</p> <p><strong>Methods:</strong> Seven hundred seventy-one patients with thyroid nodules who underwent fine needle aspiration biopsy (FNAB) and surgery at our institution were analyzed retrospectively. FNAB results&nbsp;were divided into two parts in terms of the period they related to: pre-TBSRTC (between 2005 and 2010) and TBSRTC (between 2011 and 2013).</p> <p><strong>Results:</strong> 341 FNAB were applied&nbsp;in the period of TBSRTC. Of the 341 FNAB, 53(16%) were non diagnostic, 82(24%) were benign, 62(18%) were atypia of undetermined significance or follicular lesion of undetermined significance (AUS/FLUS), 28(8%) were follicular neoplasms and/or suspicion of follicular neoplasms (FN/SFN), 95(28%) were suspicion for malignancy (SuspM), and 21(6%) were malignant. Rates of malignancy reported on follow-up histopathological examination were non diagnostic in 11%, benign in 4.9%, AUS/FLUS in 23%, FN/SFN in 32%, SuspM in 44%, and malignant in 95.3%.</p> <p><strong>Conclusions:</strong> In this study, the distribution of cases in TBSRTC categories and malignancy rates, differed from, recommended by TBSRTC and some studies. Implementation of TBSRTC did significantly affect our institution’s reporting rates.</p> 2019-06-30T11:18:55+00:00 ##submission.copyrightStatement##