TY - JOUR AU - Ekinci, Meliha AU - Akbaba, Hasan AU - Santos-Oliveira, Ralph AU - Ilem-Ozdemir, Derya PY - 2022/03/14 Y2 - 2024/03/29 TI - Development and validation of UV/VIS spectroscopy method for determination of atezolizumab in pharmaceutical products JF - EXPERIMENTAL BIOMEDICAL RESEARCH JA - Exp Biomed Res VL - 5 IS - 2 SE - Articles DO - 10.30714/j-ebr.2022275813 UR - https://www.experimentalbiomedicalresearch.com/ojs/index.php/ebr/article/view/269 SP - 175-182 AB - <p><strong>Aim:</strong> The aim of this study was to develop and validate a simple, fast, and reliable UV visible methodology for the determination of atezolizumab in pharmaceutical products.</p><p><strong>Methods: </strong>The maximum wavelength of atezolizumab was determined using a UV/Vis spectrum and the calibration curve has been established. Validation studies were carried out to determine the reliability of the spectrophotometer method used in quantification of pharmaceutical products.</p><p><strong>Results:</strong> According to the experimental data, the developed method was linear in a range varying from 0.10 to 1.50 mg.mL<sup>-1</sup> determined by 6 individuals calibrations points. The r<sup>2</sup> value was 0.9995 indicating a 99.95% correlation in linearity and precision. The robustness showed good and similar values and the limit of detection and limit of quantification were 0.005 mg.mL<sup>-1</sup> and 0.018 mg.mL<sup>-1</sup>, respectively.</p><p><strong>Conclusion:</strong> The data corroborates the reliability as applicability of the developed UV/Vis spectroscopy method for quantitatively determining the amount of atezolizumab in pharmaceutical products.</p> ER -