EXPERIMENTAL BIOMEDICAL RESEARCH https://www.experimentalbiomedicalresearch.com/ojs/index.php/ebr <p>Experimental Biomedical Research is focused on priority novel research results covering a wide range of experimental and clinical fields in the biomedical sciences</p> Prof. Dr. Hayrettin Ozturk en-US EXPERIMENTAL BIOMEDICAL RESEARCH 2618-6454 <p>Experimental Biomedical Research journal is licensed under a&nbsp;<a href="http://creativecommons.org/licenses/by-nc/4.0/" rel="license">Creative Commons Attribution-NonCommercial 4.0 International License</a>.</p> Frequency of uveitis and its associations with HLA B27 in ankylosing spondylitis patients https://www.experimentalbiomedicalresearch.com/ojs/index.php/ebr/article/view/69 <p><strong>Aim</strong>: To investigate the prevalence of uveitis in ankylosing spondylitis (AS) patients and its relationship with HLA B27.</p> <p><strong>Methods</strong>: The data of 95 patients with AS who admitted to Tokat Gaziosmanpaşa University Internal Medicine and Rehabilitation Outpatient Clinics between 2010 and 2019 were analyzed retrospectively. Demographic characteristics, HLA B27 positivity and detailed eye consultation notes of the patients were recorded.</p> <p><strong>Results</strong>: Of the 95 patients, 65 (68%) were male and 30 (32%) were female. The mean age of the patients was 38.3 ± 11.6 years. The HLA B27 results of 10 patients could not be reached. 22% of the patients had at least one episode of uveitis attack. While 82% (70/85) of patients had HLA B27 positivity, this rate was 86% in (18/21) patients with uveitis.</p> <p><strong>Conclusion</strong>: Uveitis is a disease that can cause permanent loss of vision. In our study, no significant difference was found between the presence of uveitis and the presence of HLA B27 in patients with AS and this finding needs to be supported by randomized studies.</p> Ayse Kevser Demir Hulya Deveci Selim Demir ##submission.copyrightStatement## http://creativecommons.org/licenses/by-nc/4.0 2019-09-18 2019-09-18 2 4 132 135 10.30714/j-ebr.2019454840 Evaluation of the readability of online texts related with autism spectrum disorder https://www.experimentalbiomedicalresearch.com/ojs/index.php/ebr/article/view/70 <p><strong>Aim:</strong> There has been a rapid incline in the number of patients diagnosed with autism spectrum disorder (ASD), recently. The present study aims to analyze the content and readability of online Turkish texts, on Turkish websites related to autism spectrum disorder.</p> <p><strong>Methods:</strong> In order to collect the necessary data of the present study, Google (http://www.google.com.tr), a commonly used internet search engine in our country, was used. Hence 300 websites from the first ten generated pages after writing the keywords autism, atypical autism, and autism spectrum disorder on the search engine were analyzed. Out of these 300 sites, sites containing only advertisement, videos, images and sites containing less than ten sentences, chat and forum sites, commercial sales sites and news sites that do not contain information about ASD were excluded. The mean number of syllables, the average number of words with four syllables and above, the average number of words and readability values were calculated according to Ateşman and Bezirci-Yılmaz.</p> <p><strong>Results:</strong> The mean number of words in the analyzed texts was 14.42 ± 0.48, of words with four syllables and above was 4.52 ± 0.17, of syllables was 2.92 ± 0.01 and the readability value of the texts analyzed is according to Bezirci-Yılmaz was 14.68 ± 0, 52 and according to Ateşman it was 43.72 ± 1.45. The mean number of words among the study groups is (p = 0.538), the mean number of words with four and more syllables is (p = 0.548) and the mean number of syllables is (p = 0.652), Bezirci-Yılmaz readability value is (p = 0.772), Ateşman readability value is (p = 0.626) displaying no statistically significant difference.</p> <p><strong>Conclusion:</strong> As a result of this study, it was found that the data presented on the internet about ASD was far beyond the health literacy level of our people.</p> Aziz Kara ##submission.copyrightStatement## http://creativecommons.org/licenses/by-nc/4.0 2019-09-19 2019-09-19 2 4 136 142 10.30714/j-ebr.2019454841 Evaluation of the resistance patterns of Mycobacterium tuberculosis complex strains to antituberculous drugs https://www.experimentalbiomedicalresearch.com/ojs/index.php/ebr/article/view/71 <p><strong>Aim: </strong>To determine the resistance profile of Mycobacterium tuberculosis complex (MTBC) strains to first-line antituberculous drugs.</p> <p><strong>Methods: </strong>A total of 138 patients with MTBC growth from 2008-2018 were evaluated retrospectively. The Ehrlich-Ziehl-Neelsen (EZN) staining method was used for direct smear preparations, the BACTEC MGIT 460 TB system the Lowenstein-Jensen medium for culture planting and the BACTEC NAP test for the diagnosis of MTBC. Susceptibility tests were performed using the BACTEC MGIT 460 TB system with the streptomycin, isoniazid, rifampicin and ethambutol (SIRE) kit in accordance with the manufacturer's recommendations.</p> <p><strong>Results: </strong>Of the total 138 tuberculosis (TB) cases, 44 (31.9%) were female and 94 (68.1%) were male. MTBC was most frequently isolated from pulmonary specimens (90.6%). Acid-resistant bacilli (ARB) positivity was detected in 88 (63.8%) samples by EZN staining for culture-positive samples. In our study, without considering single or multiple drug resistance (MDR), total resistance rates in MTBC strains were determined for, isoniazid (INH), rifampicin (RIF), ethambutol (EMB), and streptomycin (SM); 10.1%, 4.3%, 2.9%, and 12.3% respectively. While the susceptibility to all drugs was 82.6%, multiple drug–resistant tuberculosis (MDR-TB) was 2.9%.</p> <p><strong>Conclusion: </strong>These results are important since they are the first data reported from our province regarding the determination of the resistance profile to anti-TB drugs. Resistance rates in our study were very close to the 2016 data average of the Ministry of Health of Turkey. Determination of TB resistance profiles, as well as proper and regular treatment, will contribute to the control of MDR-TB.</p> Mustafa Behcet Seyda Karabork Fatma Avcioglu Ayse Karaibrahim Muhammet Guzel Kurtoglu ##submission.copyrightStatement## http://creativecommons.org/licenses/by-nc/4.0 2019-09-19 2019-09-19 2 4 143 148 10.30714/j-ebr.2019454842 Protective effect of thymoquinone on smoking-induced vascular damage: An experimental study in rats https://www.experimentalbiomedicalresearch.com/ojs/index.php/ebr/article/view/72 <p><strong>Aim:</strong> To investigate the protective effect of thymoquinone on smoking-induced vascular damage in rats.</p> <p><strong>Method:</strong> A total of 28 rats were allocated in this experimental study. Rats were equally divided in four groups; as control group (n=7) and study groups including only smoking group (n=7), smoking plus thymoquinone group (n=7) and smoking plus dexamethasone group (n=7). The animals in study groups were subjected to regular cigarette smoke exposure during 2 months, hereby smoking-induced vascular injury model was established in the animals. The thoracic aorta of the animals were surgically resected and then evaluated histopathologically. The prepared aortic tissue samples were analyzed under light microscope in terms of thickness of aortic wall, endothelial continuity and injury as well as degenerative alterations on the endothelium.</p> <p><strong>Results:</strong> Mean aortic wall thicknesses were 77.8 and 114.5 μ in only smoking group and smoking plus thymoquinone group respectively, and this difference was statistically significant. In only smoking group partial endothelial damage and complete endothelial damage were observed in 2 and 4 animals respectively whereas in smoking plus thymoquinone group no endothelial damage and partial endothelial damage were observed in 2 and 4 animals respectively. When these groups were compared in terms of endothelial damage, the difference was detected to be statistically significant.</p> <p><strong>Conclusion:</strong> Our study demonstrates that thymoquinone has a protective effect on rat endothelium and alleviates the smoking-induced vascular damage in rats.</p> Yusuf Velioglu Ahmet Yuksel Osman Yaksi Songul Peltek Ozer Mehmet Unal Emine Ozsari Erhan Renan Ucaroglu ##submission.copyrightStatement## http://creativecommons.org/licenses/by-nc/4.0 2019-09-19 2019-09-19 2 4 149 157 10.30714/j-ebr.2019454852 Five-year experience of neurosyphilis cases in a tertiary care hospital in Turkey https://www.experimentalbiomedicalresearch.com/ojs/index.php/ebr/article/view/76 <p><strong>Aim: </strong>To evaluate retrospectively the cases of syphilis who applied to different clinics of our hospital in the last 5 years and to emphasize the importance of admission screening tests in the diagnosis of neurosyphilis which is a rare but serious form of syphilis.</p> <p><strong>Methods: </strong>Sixty-one patients who admitted to different clinics of our hospital between 2013 and 2017 with different clinical manifestations and positive Venereal Disease Research Laboratory (VDRL) and Treponema pallidum microhemagglutination assay (TPHA) tests were recorded and analyzed retrospectively.</p> <p><strong>Results: </strong>Both VDRL and TPHA tests were positive in 44 males with a mean age of 57.4 ± 18.4 years and 17 females with a mean age of 65.3 ± 19.1 years. It was found that of the 61 patients who were examined for differential diagnosis of syphilis, 36 (%59) of them were referred from neurology clinic, 15 (25%) from infectious diseases clinic, 7 (12%) from dermatology clinic and 3 (4%) from gynecology clinic.&nbsp;&nbsp; The clinical presentation was venereal chancre in 15 cases, asymptomatic ischemic brain lesions in 17 cases, acute ischemic stroke in 13 cases, movement disorder in three cases, tabes dorsalis in two cases, tendinitis-arthritis in eight cases, Amyotrophic lateral sclerosis (ALS) in one case, Deep venous thrombosis (DVT) in one case, and pulmonary nodule in one case.&nbsp;</p> <p><strong>Conclusions: </strong>In conclusion, syphilis is often overlooked in the differential diagnosis in patient with neurological findings. Since neurosyphilis is an easily treatable disease, it should be considered in the differential diagnosis of patients presenting with neurological findings.</p> Muhammed Nur Ogun Fatma Sirmatel ##submission.copyrightStatement## http://creativecommons.org/licenses/by-nc/4.0 2019-09-19 2019-09-19 2 4 158 162 10.30714/j-ebr.2019454853 Does hemogram biomarkers predict mortality in intensive care population? https://www.experimentalbiomedicalresearch.com/ojs/index.php/ebr/article/view/74 <p><strong>Aim: </strong>To compare hemogram-mediated inflammatory markers such as mean platelet volume (MPV), red cell distribution width (RDW), platelet distribution width (PDW), neutrophil to lymphocyte ratio (NLR), platelet to lymphocyte ratio (PLR), MPV to platelet ratio (MPR) and RDW to platelet ratio (RPR) in predicting outcome in the intensive care units (ICU) population.</p> <p><strong>Methods</strong>: After obtaining approval of intuitional board the patients whom were treated in the Anesthesiology and Reanimation ICU of Bolu Abant Izzet Baysal University Hospital between March 2017 and December 2018 were evaluated retrospectively and enrolled to the study. The above-mentioned laboratory parameters of the deceased and survivors were compared.</p> <p><strong>Results</strong>: The PDW, MPV, C-reactive protein (CRP), RPR and MPR values were significantly different between deceased and survived subjects. Moreover, MPV and PDW values ​​were significantly and positively correlated with serum CRP level.</p> <p><strong>Conclusion</strong>: We suggest that PDW, MPV, MPR and RPR could be used in determining the severity of the disease in ICU patients along with CRP. Since these hemogram results could be obtained in a much shorter time period compared to CRP, they could be useful in this population; in which hours and even minutes are important in the treatment in ICU.</p> Ibrahim Karagoz ##submission.copyrightStatement## http://creativecommons.org/licenses/by-nc/4.0 2019-09-19 2019-09-19 2 4 163 168 10.30714/j-ebr.2019454854 Bacterial etiology and antimicrobial susceptibility profiles of 7741 urine cultures in outpatients: A 5-year single-center experience in Turkey https://www.experimentalbiomedicalresearch.com/ojs/index.php/ebr/article/view/75 <p><strong>Aim: </strong>To determine the epidemiological and antibiotic <strong>s</strong>usceptibility profile of bacterial uropathogens in outpatients.</p> <p><strong>Methods:</strong> This study analyzed data taken from outpatients with urinary tract infection (UTI) collected from January 2013 to January 2018 in the laboratory of Maltepe Medical Center in İstanbul. Species of uropathogens were made using routine laboratory methods. Antibiotic resistance profile of uropathogens was determined using disk diffusion method.</p> <p><strong>Results</strong>: There were 7741 urine cultures and 1563 of them were positive for bacterial uropathogens. The identified major organisms were Gram-negative bacteria (1376/1563; 88.0%), while Gram-positive bacteria only 5.4% (187/1563) were representing. The patients with positive samples, 84.8% (1326/1563) were female and 15.1% (237/1563) were male. The main isolated uropathogen was <em>E. coli</em> (58.1%), followed by <em>Enterobacter spp</em> (10.1%), <em>P. mirabilis</em> (8.6%), <em>K. pneumoniae</em> (7.8%),<em> Enterococcus </em>(4.7%)<em> and P. aeruginosa</em> (3.1%).<em> E. coli </em>was more prevalent in all age groups. A total of 74.8% of <em>E. coli</em> isolates were resistant to ampicillin and the lowest resistance rate was to imipenem (0.3%). <em>Enterobacter species </em>were found to have higher resistance to ampicillin (87.3%), lower resistance to carbapenem (1.8%) and amikacin (6.9%).</p> <p><strong>Conclusion</strong>: Due to the emergence of various antibiotics resistant bacterias, it was concluded that empirical antibiotic treatment should be reviewed periodically according to the regions. Empirical therapy must be based on local epidemiological data, which should be constantly updated. Therefore, the results of our study may help physicians to select an appropriate antimicrobial therapy.</p> Orhan Akpinar Mustafa Guzel ##submission.copyrightStatement## http://creativecommons.org/licenses/by-nc/4.0 2019-09-19 2019-09-19 2 4 169 178 10.30714/j-ebr.2019454855